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Table 2 Results of Z -tests for selection on fish owl MHC class IIβ sequences

From: Spatial and temporal variation at major histocompatibility complex class IIB genes in the endangered Blakiston’s fish owl

 

d S

d N

Z

P

(A) All alleles

    

PBR

0.139 ± 0.059

0.464 ± 0.091

3.563

<0.001

Non-PBR

0.167 ± 0.040

0.095 ± 0.020

−1.644

1.000

All sites

0.158 ± 0.031

0.179 ± 0.028

0.309

0.499

(B) Expressed alleles

   

PBR

0.109 ± 0.049

0.404 ± 0.081

3.100

0.001

Non-PBR

0.114 ± 0.033

0.058 ± 0.016

−1.579

1.000

All sites

0.112 ± 0.026

0.139 ± 0.026

0.801

0.212

(C) Unexpressed alleles

   

PBR

0.161 ± 0.084

0.468 ± 0.105

2.589

0.005

Non-PBR

0.236 ± 0.074

0.127 ± 0.029

−1.413

1.000

All sites

0.213 ± 0.052

0.206 ± 0.034

−0.124

1.000

  1. Z-test for selection was conducted with (A) all alleles together, (B) only with expressed alleles, and (C) only with unexpressed alleles (see Figure 2 and [Additional file 1: Table S1]). The average rates of synonymous (d S) and non-synonymous substitution (d N) were calculated for all sites, as well as for positions encoding amino acids in the putative peptide-binding region (PBR), and the remaining positions (putative non-PBR). Standard errors obtained through 1000 bootstrap replicates. The location of the PBR was inferred from the molecular structure of human leukocyte antigen class II, HLA-DR1 [54].
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Zoological Letters

ISSN: 2056-306X

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