Table 2 PCR primers newly designed in the present study for amplification and/or sequencing for the Blakiston’s fish owl mtDNA control region
Primer | Nucleotide sequence (5′–3′) | Citation |
|---|---|---|
L16728 | CCAAGGTCTGCGGCTTGAAAAG | Omote et al. [12] |
Hcontrol-03-kb | TGAAGAGTTATGGTTTAGGTACG | Omote et al. [12] |
Hcontrol-05-kb | GGGCATTAATGTCATGAAATTAG | This study |
Lcontrol-06-kb | TACTAATCCATGCACTAATCCC | This study |
Hcontrol-07-kb | GCCATGGATTGGAGTATTAATAG | This study |
Lcontrol-08-kb | CAGTTGTACATTAAACCATCTAC | This study |
Hcontrol-09-kb | GGCATGGATGTTATATCTTGGTG | This study |
Lcontrol-12-kb | GTACTAATCACATACAATTCATG | This study |
Lcontrol-14-M13f | GTAAAACGACGGCCAGCCATTAATGTGCTACGTATATAC | This study |
Hcontrol-15-M13f | GTAAAACGACGGCCAGATGGGATTAGTGCATGGATTAG | This study |
Lcontrol-16-M13f | GTAAAACGACGGCCAGCCTATTCATGACAGAACGACA | This study |