Fig. 1

A phylogenetic tree based on the FP gene sequences. The tree was constructed using the maximum likelihood method. The bootstrap probability (>60) for each clade was obtained by 1,000 replicates and is shown next to each node. The evolutionary distances were computed using the p-distance method. All sites containing gaps and missing data were eliminated from the sequences used in the analysis. There were a total of 333 nucleotide sites in the final dataset. Evolutionary analysis was conducted in MEGA5. The monGFP is shown in gray. The scale bar represents 0.05 substitutions per site. GFP from Aequorea victoria was used as an outgroup. The accession numbers and species name for the FP sequences are as follows: Acropora digitifera, AdiFP1 BR000962, AdiFP2 BR000963, AdiFP3 BR000964, AdiFP4 BR000965, AdiFP5 BR000966, AdiFP6 BR000967, AdiFP7 BR000968, AdiFP8 AB698751, AdiFP9 BR000969, AdiFP10 BR000970; Montipora millepora, meffCFP DQ206381, mmilCFP DQ206392, Montipora efflorescens, meffCP DQ206377, meffGFP DQ206393, meffRFP DQ206379; Psammocora sp., psamCFP EU498721; and Aequorea victoria GFP M62653