Fig. 3

Splicing variants of monGFP (a) RT-PCR amplification of monGFP mRNAs from Montipora sp. M and C indicate molecular marker øX174/HaeIII and the negative control, respectively. The PCR products for each type of mRNA are marked by arrows. (b) A nucleotide alignment of the 5’ end of monGFP variants (M5_S type and M5_L type) with RFPs (amilRFP and meffRFP). The first and second initiation codons (ATG) are highlighted in black. The additional sequence in M5_L type cDNA is underlined. Exons 1 and 2 are shown in gray. The position of upstream primer (M5_F1) is shown by bold line. Schematic representation of the position of the additional sequence and splicing of S and L types is shown in lower panel. (c) Schematic representation of monGFP variants. Nucleotide differences among variants are shown by gray (minor alleles) and black (major alleles) bars. The alphabetical characters indicate nucleotides (above the box) and amino acids (below the box). The first and second initiation codons are shown as “ATG” in the box. The positions of exons 1 and 3 are shown at the bottom