Fig. 4

The structure of monGFP genes (a) PCR amplification of partial (left) and full-length (right) of monGFP intron 1 from the genomic DNA of Montipora sp.. M1, M2 and C indicate molecular markers λ/HindIII, øX174/HaeIII and negative control, respectively. The PCR products of each intron 1 sequence are marked by arrows. b Schematic representation of the structure of monGFP genes. Nucleotide differences among variants are shown by bars. The alphabetical characters indicate nucleotides (above the boxes). Dotted boxes indicate unidentified exons. The approximate lengths of introns are shown below the introns. Positions of primers used in PCR amplification to determine genomic structures are indicated by arrows