Fig. 6

Immunohistochemistry of the testes using specific antibody of three steroidogenic enzymes. a, d P450 cholesterol side chain cleavage (SCC). b, e 3β-hydroxysteroid dehydrogenase (3β-HSD). c, f P450 aromatase (Arom). a-c Control fish. d-f Sterilized fish. Clusters of immunopositive cells against SCC and 3β-HSD were detected in the interstices in both sterilized and normal testes (a, b, d, e). No positive cells against Arom were observed in the testes of fish from both groups c, f