Fig. 3

External morphology of Xenopus larvae. Blue staining show nucleus labeled by DAPI. Myotomes (a–c) and nerves (d–s) are visualized by immunohistochemistry (shown in green). a–c Lateral view of the trunk in un-injected control (a), slit2-MO-injected (b) and robo2-MO-injected (c) specimens. Development of myotomes is normal in all conditions. d–g Lateral view of the trunk in slit2-control-MO-injected (d), slit2-MO-injected (e), robo2-control-MO-injected (f), robo2-MO-injected (g) specimens. Arrowheads indicate segmentally organized spinal nerves. h–k Dorsal view of the anterior trunk in slit2-control-MO-injected (h), slit2-MO-injected (i), robo2-control-MO-injected (j), robo2-MO-injected (k) specimens. Arrowheads indicate segmentally organized spinal nerves. l-o Dorsal view of the head in slit2-control-MO-injected (l), slit2-MO-injected (m), robo2-control-MO-injected (n), robo2-MO-injected (o) specimens. p-s Dorsal view of the optic and cranial nerves in slit2-control-MO-injected (p), slit2-MO-injected (q), robo2-control-MO-injected (r), robo2-MO-injected (s) specimens. Arrowheads indicate the optic nerves. The peripheral nerves visualized by immunohistochemistry represent a normal innervation pattern. Scale bars: A–K, P-S, 200 μm; L-O, 500 μm