Amphioxus mouth after dorso-ventral inversion

Zoological Letters

Table 1 List of forward and reverse primer sets for PCR amplification

Name of gene	Forward primer	Reverse primer	Accession no.	Reference
dkk1/2/4	CCGTAACTTGTCCACACCGTAGA	GTGCTTTGATGCGTCTTGTTGGG	HM590023	Zhang and Mao (2010) [35]
frzb1 ^a)	GTGGTTCCTCCCGTTGTTG	GCTTCAGCCGTCTTTCCCA	XM002612836	Putnam et al. (2008) [52]
lim1/5 ^b)	AGGGACTCCAAACTGTACTG	TTACCACACCGACTCTTCC	DQ399521	Langeland et al. (2006) [30]
mef2 ^c)	GCTTATGAGCTGAGTGTGCT	GGTTCACTCTTGATCTGCAT	EF407505	Zhang et al. (2007) [53]
pax2/5/8	TGTGACAACGACACAGTTCC	AACAGCAACTGGATAGTGGC	AB193513	Hiruta et al. (2005) [54]
pax3/7 ^d)	GGTGGAGAAGAAGATAGAGG	ACGGATGTTCAGTACGCTTG	XM002610760	Holland et al. (1999) [55]
pou4 ^e)	ACAACACATCAGCATGCACC	GGTTTTGTTTTGACCGTTATT	DQ314242	Candiani et al. (2006) [28]

Sequences of Branchiostoma japonicum gene fragments were registered under accession numbers ^a)AB979875, ^b)AB980198, ^c)AB980199, ^d)AB980200, and ^e)AB980202

ISSN: 2056-306X