Fig. 3

Double-plotted actograms of locomotor activity of adult male crickets Gryllus bimaculatus under LD 12:12, LD 20:4, and DD. (a) intact cricket, (b) cricket treated with dsDsRed2, (c, d) crickets treated with dsGb’E(z). Crickets were exposed to LD 12:12 for the first 5 days, then in LD 20:4 for 9 days, and transferred to DD on the day indicated by an arrowhead, at a constant temperature of 25 ± 0.5 °C. α and ρ indicate active phase and rest phase, respectively. Dotted lines indicate onset and offset of the active phase. White and black bars above the actogram indicate the light and dark phases, respectively. Gray area in the actogram indicates the dark phase