Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus

Table 1 Effects of RNAi against Gb’E(z) and DsRed2 on the locomotor rhythm of Gryllus bimaculatus

Treatment	n	Light Condition	τ (mean ± SD) h	α/ρ ratio (mean ± SD)
LD12:12
intact	13	LD	-	0.94 ± 0.07^a
	12	DD	23.66 ± 0.25^a	0.95 ± 0.23^a
DsRed2 ^RNAi	23	LD	-	0.97 ± 0.09^a
	18	DD	23.63 ± 0.33^a	0.97 ± 0.11^a
Gb’E(z)^RNAi	26	LD	-	0.99 ± 0.08^a
	23	DD	23.97 ± 0.16^b	1.02 ± 0.11^a
LD20:4
intact	23	LD	-	0.53 ± 0.20^cd
	13	DD	23.80 ± 0.12^ab	0.46 ± 0.20^d
DsRed2 ^RNAi	33	LD	-	0.69 ± 0.22^bc
	22	DD	23.70 ± 0.23^a	0.55 ± 0.19^cd
Gb’E(z)^RNAi	23	LD	-	0.84 ± 0.28^ab
	17	DD	23.99 ± 0.16^b	0.83 ± 0.25^ab

ANOVA revealed significant difference in both free-running period (τ) (F_{5, 98} = 9.01, P < 0.01) and α/ρ ratio (F_{11, 230} = 22.0, P < 0.01). Values with different lower case letters significantly differ from each other (Tukey-test, P < 0.01, except for α/ρ ratios between intact in DD following LD 20:4 and DsRed2 ^RNAi in LD 20:4, where P < 0.05)

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