Fig. 3
From: Mechanisms of wound closure following acute arm injury in Octopus vulgaris
A cellular plug rapidly covers exposed tissue. (a, b) H&E and c, d Periodic Acid-Schiff staining of healing amputation wounds. An increasing cellularity of the wound bed was seen during the time-course studied. PAS confirmed that this is not simply mucous secretions. Arrow overlies central tissue and highlights width of wound. WE, wound-edge epithelium; WB, wound bed. e Scanning EM showed diverse sub-cellular-sized structures on the surface of the plug (e.g. extracellular vesicles, mucus). f Transmission EM revealed the densely packed and tightly interdigitated cells (arrowheads show finger-like processes of cell membrane) comprising the deeper plug. Scale bars: a&b, 200 μm; c&d, 50 μm; e, 5 μm; f, 10 μm