Fig. 4From: Past seawater experience enhances seawater adaptability in medaka, Oryzias latipes a, b Double immunofluorescence staining for NKA (green) and CFTR (magenta) in the gills of FW (a)- and SW (b)-acclimated medaka. c–e The density of ionocytes (c), ratio of CFTR-immunoreactive ionocytes to NKA-immunoreactive ionocytes (d), ratio of multicellular complex ionocytes to total ionocytes (e) and size of ionocytes (f) in the gills of FW- and SW-acclimated medaka. Values are means ± SEM (n = 6). Asterisks indicate significant differences between the two groups (two-sided Student’s t test, *P < 0.05 and ***P < 0.001)Back to article page