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Table 3 Gene expression of various NKA α-subunits quantified by RNA-seq in the gill and intestine of Japanese medaka and eel. RBBH Reciprocal BLAST Best Hits

From: Flexible selection of diversified Na+/K+-ATPase α-subunit isoforms for osmoregulation in teleosts

Medaka Ensembl accession no.

   

RNA-seq (reads per million)

Eel

RNA-seq-RBBH genes

Annotation

Identity

 

Gill (N = 3)

Intestine (N = 5)

Identity

Gill (N = 3)

Posterior Intestine (N = 5)

   

Salinity

Mean

±

SD

Mean

±

SD

 

Salinity

 

Mean

±

SD

ENSORLG

atp1a1a.4

α1a

FW

13973

±

11507

41

±

29

No entry

FW

nil

nil

  

00000018557

(2 of 2)

   

50 % SW 7d

18306

±

1414

61

±

15

 

SW 7d

nil

nil

  

ENSORLG

atp1a1a.4

α1b

FW

44604

±

23376

56974

±

11754

No entry

FW

nil

nil

  

00000002122

(1 of 2)

   

50 % SW 7d

85481

±

14483

52584

±

2749

 

SW 7d

nil

nil

  

ENSORLG

atp1a1b

α1c

FW

4948

±

948

941

±

117

α1c

FW

nil

2094

±

483

00000002047

   

50 % SW 7d

5034

±

437

952

±

152

 

SW 7d

nil

1599

±

695

ENSORLG

atp1a2a

α2

FW

605

±

313

2

±

1

No entry

FW

nil

nil

  

00000002639

   

50 % SW 7d

348

±

70

5

±

10

 

SW 7d

nil

nil

  

ENSORLG

atp1a3a

α3a

FW

91

±

46

20

±

8

α3

FW

nil

63

±

55

00000007036

   

50 % SW 7d

92

±

14

12

±

13

 

SW 7d

nil

40

±

24

ENSORLG

atp1a3b

α3b

FW

14354

±

571

318

±

99

No entry

FW

nil

nil

  

00000013191

   

50 % SW 7d

21286

±

2246

241

±

70

 

SW 7d

nil

nil