Fig. 7

serotonin-LIR nervous system of Diuronotus aspetos. The anterior is pointing left for all figures. a, b Schematic drawings of the serotonin-LIR of the anterior part of the specimen: nerves and perikarya in green, nuclei in grey, and opposite ventral or dorsal nervous system in light grey. a Dorsal view, b ventral view. c–f CLSM images with serotonin-LIR in glow. c CLSM maximum intensity projection (MIP) of the entire specimen. d Dorsal MIP of the brain e CLSM sub-stack MIP showing details of the brain perikarya f CLSM sub-stack MIP of the ventro-posterior terminal part of the specimen. br brain, ph pharynx, sacn serotonin-LI-reactive anterior commissure of the neuropil, sanr serotonin-LI-reactive anterior nerve ring, sdlp serotonin-LI-reactive dorso-lateral perikaryon, sdmp serotonin-LI-reactive dorso-median perikaryon, slbn serotonin-LI-reactive lateral brain nerve, slnc serotonin-LI-reactive ventro-lateral nerve cord, slpn serotonin-LI-reactive lateral nerves of the posterior commissure of the neuropil, spln serotonin-LI-reactive postero-lateral nerve node, smbn serotonin-LI-reactive median-most brain nerve, smcn serotonin-LI-reactive median commissure of the neuropil, snp serotonin-LI-reactive neuropil, snpt serotonin-LI-reactive nerve projection of the primary tube, spag serotonin-LI-reactive perikarya of the anal ganglion, spbn serotonin-LI-reactive paramedian brain nerve, spcn serotonin-LI-reactive posterior commissure of the neuropil, spco serotonin-LI-reactive posterior commissure, spog serotonin-LI-reactive perikarya of the post-pharyngeal ganglion, spp serotonin-LI-reactive neuropil patch, sppg serotonin-LI-reactive para-pharyngeal cluster