Fig. 3

Immunofluorescence analysis. Identity numbers for each mutants are shown as #09–11. a, b Lateral bifurcated mutants. c A symmetrically bifurcated mutant. Nerve fibers and pronephric ducts are visualized by anti-acetylated tubulin antibody in magenta and cell nuclei are labeled by YOYO-1 in green in (a) and (c). In (b), (c’) and the inset in (a), only the signal of anti-acetylated tubulin antibody is shown. Scale bars: 200 μm