Fig. 2

A diagram showing the process for selecting candidates of genes associated with ovulation by the Subio platform (Subio Inc., Amami, Japan). In standard statistical selection, ANOVA was performed across all groups to detect significant differences in both up- and down-regulation among all treated groups (P ≤ 0.05) (a). More than 1.8-fold greater expressions in 17, 20β-DHP than in EtOH (1) DES (2) or Tes (3) have been reported. Overlapping genes among these three groups were selected by Venn diagrams (b). Finally, overlapping genes in categories A and B were selected. Thirty-three genes were selected as candidates for genes associated with ovulation. Twenty highly elevated genes analyzed by qPCR were listed in Table 1. For non-statistical gene selection, more than 10-fold greater expressions in the 17, 20β-DHP-treated sample compared with the EtOH- (4) DES- (5) or Tes- (6) treated samples were analyzed by Venn diagrams. Two genes were selected as candidates for genes associated with ovulation (Table 2)