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Fig. 3 | Zoological Letters

Fig. 3

From: Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish

Fig. 3

qPCR analysis of candidate genes associated with ovulation listed in Tables 1 and 2 with ptgs2a as a positive control was conducted using cDNAs prepared from ovaries from fish treated by EtOH, DES, Tes or 17, 20β-DHP for three hours. The mRNA abundance was observed in triplicate for each sample and all data were normalized with the number of transcripts of elongation factor 1α (EF1α) in each sample. Expression values are represented as the mean ± SE of three independent samples. Different letters represent significant differences among the data (P ≤ 0.05)

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