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Fig. 6 | Zoological Letters

Fig. 6

From: cryptochrome genes form an oscillatory loop independent of the per/tim loop in the circadian clockwork of the cricket Gryllus bimaculatus

Fig. 6

Suppressive effects of Gb’CRY1 or Gb’CRY2 proteins on tim transcription. Relative luciferase activity of tim-luc was measured in the presence of 100 ng of pAc-Clk with various combinations of the indicated pAc5.1B-Gb’cry1, pAc5.1B-Gb’cry2a, -b, -c, -d, -e, or -f expression plasmids. Signals were normalized to Rluc activity and then to the control signal of cells transfected with 100 ng of tim-luc and pAc-Clk. Error bars represent SEM (n = 3). A combination of Gb’cry2b and Gb’cry2c suppressed the transcription of E-box genes. Asterisks represents P < 0.01 versus control (Dunnett’s multiple range test)

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