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Table 1 Primers used for qPCR, PCR, dsRNA synthesis, and cloning of coding regions for cell-based assay

From: cryptochrome genes form an oscillatory loop independent of the per/tim loop in the circadian clockwork of the cricket Gryllus bimaculatus

Primers

Forward

Reverse

For qPCR

Gb’cry1

5′-AAAGCCAGTTACGCTGATGG-3′

5′-TGTAATCTCAGGCCATGTCG-3′

Gb’cry2

5′-AGCACCATCACACACTTCACA-3′

5′-ACACTCAGCGCAATCCACAC-3′

Gb’per

5′-AAGCAAGCAAGCATCCTCAT-3′

5′-CTGAGAAAGGAGGCCACAAG-3′

Gb’tim

5′-CCAAAGACAGGAAGCAGACC-3′

5′-GAATCCCAACACCAAAATGG-3′

Gb’rpl18a

5′-GCTCCGGATTACATCGTTGC-3′

5′-GCCAAATGCCGAAGTTCTTG-3′

For PCR

Gb’cry2-x2a

5′-GTGGTATCAACGCAGAAGTA-3′

5′-TCTAGGCACTGTAGTAGGAA-3′

Gb’cry2-x4

5′-TTCCGATGCATTTTCATCC-3′

5′-CGCCAAAGCTTCTGATTCTCC-3′

Gb’cry2-x10b

5′-CTCCTGAGAGTGTGCAACGT-3′

5′-ACTTGATGGAACTGCTGCCA-3′

For dsRNA synthesis

Gb’cry1#d1

5′-TAATACGACTCACTATAGGGGTTTCGACATGGCCTGAGAT-3′

5′-AATTAACCCTCACTAAAGGGCTTCTGGCGTTGGAAAATGT-3′

Gb’cry1#d2

5′-TAATACGACTCACTATAGGGTGGGAACAAGGAAAGACTGG-3′

5′-AATTAACCCTCACTAAAGGGCGACAATGAGGTGGAGGATT-3′

Gb’cry2#d1

5′-TAATACGACTCACTATAGGGCTGCGACAAATAACCCCAAC-3′

5′-AATTAACCCTCACTAAAGGGCTCTCAGGAGCATTCCAAGG-3′

Gb’cry2#d2

5′-TAATACGACTCACTATAGGGAAGCACACTGTGCATTGGTT-3′

5′-AATTAACCCTCACTAAAGGGCCGTTCTTTTCGATGATGCT-3′

Gb’per

5′-TAATACGACTCACTATAGGGCATTCATCGACTTCGTTCACC -3′

5′-AATTAACCCTCACTAAAGGGCTGAACGCCCAATCATGTCT -3′

Gb’tim

5′-AATTAACCCTCACTAAAGGGGTAAAGAAGATAGAGAGTAT-3′

5′-AATTAACCCTCACTAAAGGGTTGGAGAGAACTGAAGAGGT-3′

Gb’Clk

5′-TAATACGACTCACTATAGGGTACATCACGCCATAGCCTTG -3′

5′-AATTAACCCTCACTAAAGGGGGGATTGCTCTTCTTTGCTG -3′

Gb’cyc

5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′

5′-AATTAACCCTCACTAAAGGGAGGTTCTGCTGCTTCTTTCG-3′

DsRed2

5’-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′

5′-TAATACGACTCACTATAGGGCTACAGGAACAGGTGGTGGC-3′

For cloning of coding region for cell-based assay

Gb’cry1

5′-ATGCAGGTACCAAGCCAGTTACGCTGATGGT-3′

5′-GGGCTCGAGAGTGGTCAAAGCAATTATCAT-3′

Gb’cry2#p1

5′-ATGCAGGTACCATGGGAAGCACACTTGCATT-3′

5′-ATGCAGCGGCCGCAATTGTGCTGTTGATTTAAAC-3′

Gb’cry2#p2

5′-ATGCAGGTACCAGTGCTCGTGTGTGTGTTTG-3′

5′-ATGCAGCGGCCGCAATTGTGCTGTTGATTTAAAC-3′

  1. Gb’cry1, Gb’cry2#p1, Gb’cry2#p2 were used for cloning of Gb’cry1, Gb’cry2a ~ c, and Gb’cry2d ~ f, respectively