Novel bacteriocyte-associated pleomorphic symbiont of the grain pest beetle Rhyzopertha dominica (Coleoptera: Bostrichidae)

Table 1 Relative rate tests of 16S rRNA gene sequences of the symbiont of Rhyzopertha dominica in comparison with Sulcia symbionts and Blattabacterium symbionts

Lineage 1	Lineage 2	Outgroup	K1^a	K2 ^b	K1-K2	K1/K2	P value ^c
Symbiont of Rhyzopertha dominica [LC310894]	Sulcia symbionts ^d	Flavobacterium columnare [AB010952]	0.150	0.076	0.074	2.0	2.5 × 10⁻⁶
Symbiont of Rhyzopertha dominica [LC310894]	Blattabacterium symbionts ^e	Flavobacterium columnare [AB010952]	0.159	0.059	0.100	2.7	1.0 × 10⁻⁷
Sulcia symbionts ^d	Blattabacterium symbionts ^e	Flavobacterium columnare [AB010952]	0.083	0.056	0.027	1.5	0.01

^aEstimated mean distance between lineage 1 and the last common ancestor of lineages 1 and 2
^bEstimated mean distance between lineage 2 and the last common ancestor of lineages 1 and 2
^c P-value was generated using the program RRTree [50]. The analyses were performed using 1445 aligned nucleotide sites for 16S rRNA gene sequences
^d16S rRNA sequences of Sulcia symbionts from Homalodisca vitripennis (CP000770), Diceroprocta semicincta (CP001605) and Clastoptera arizonana (CP002163)
^e16S rRNA sequences of Blattabacterium symbionts from Blattella germanica (CP001487), Mastotermes darwiniensis (CP003000), and Cryptocercus punctulatus (CP003015)

ISSN: 2056-306X