Fig. 3

Effects of cholesterols on medaka RORd1. a Effects of cholesterols on medaka RORd1 transcriptional activity, assessed by luciferase reporter assay; stably transfected cells were treated with vehicle (control) or 10 μM of cholesterol derivatives. The white column is vehicle (ethanol), and the hatched and black columns represent ROR inverse agonists and agonists in mammals, respectively. Longitudinal axis shows relative luminescence units (RLU). Statistical differences between vehicle and ligands are indicated by asterisks (**: P < 0.01; ***: P < 0.001). 7α-hydroxycholesterol (7αOHC); 7β-hydroxycholesterol (7βOHC); 7-ketocholesterol (7ketoC); 22(R)-hydroxycholesterol (22RHC); 20α-hydroxycholesterol (20αHC); 25-hydroxycholesterol (25HC). b Concentration-response curve of 20αHC. Broken line shows the luminescence of ethanol-treated cells. Each point represents the mean of triplicate determinations, and vertical bars represent the S.E.M (a, b)