Fig. 5
Reporter gene assay using consensus RRE in bmal1a or cyp26a1 promoter of medaka and these expression rhythms. HEK293 cells transfected with (a) bmal1ap-luc or (b) cyp26a1p-luc and pcDNA-RORd1 or pcDNA were dosed with EtOH (open) or 10−5 M 4OH-ATRA (closed). Longitudinal axis shows relative luminescent units (RLU). The temporal change in expression of (c) bmal1a and (d) cyp26a1 in eyes. The sample collection time is indicated as ZT. White and black bars above each graph represent light and dark periods. Y-axes represent gene expression as copies/μg-total RNA. Data are expressed as means ± standard error of the mean (S.E.M.) (n = 6). Different letters on the columns indicate group means that are statistically different when analyzed using one-way ANOVA followed by Tukey’s Multiple Comparison Test (P < 0.001)