Fig. 2
From: A sodium binding system alleviates acute salt stress during seawater acclimation in eels
Immunohistochemistry of NKCC and CFTR in the gill and anterior intestine (AI) of eels in FW and time course SW exposure. Apical CFTR signals (arrow heads) on the ionocytes at the interlamellar region are not observed from FW to SW 3 d (a-e), and become apparent on the ionocytes of SW 7 d eels (f). Basolateral NKCC (arrows) in ionocytes are scarcely observed from FW to SW 1 d (g-j), and become increasingly abundant in numbers and intensity from SW 3 d to 7 d (k-l). Apical NKCC (arrows) on the intestinal epithelia are present in all time course samples (m-r), but an increasing intensity is observed in SW 3 d to 7 d. Scale bar = 100 μm