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Fig. 7 | Zoological Letters

Fig. 7

From: A sodium binding system alleviates acute salt stress during seawater acclimation in eels

Fig. 7

Partial purification of Na-binding molecules from esophageal and intestinal luminal secretion using gel filtration HPLC. a A representative chromatograph (OD 280 nm) showing the separation of a FW esophageal luminal secretion sample (grey, left Y-axis). The gel filtration column is equilibrated with 0.1 M Tris-HCl (pH 7.4) at a flowrate of 0.5 mL/min. Large proteins (> 4 kDa) and free Na are eluted at the flow through (10–15 min). The Na content of each 2 min fraction (black, right Y-axis) was superimposed on the chromatograph to indicate the relationship among molecular size, protein quantity, and Na-binding capacities. A bound-Na peak is present with molecular size ~1.8 kDa. b Effects of 3 h SW transfer on the total bound Na in the luminal secretions of eel esophagus and intestine (N = 5). Total bound Na is defined as the sum of Na contents in non-flow-through fractions in A). Statistical difference between salinity treatment in the same tissue is indicated by * (p < 0.05) after two way ANOVA, Bonferroni’s test

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