- The protospacer-adjacent motif (PAM) sequence is labeled in green. The bold letters indicate that the corresponding sgRNAs were used to generate knock-in transgenic fish. The lowercase letter, g, at the beginning of the sequences indicates that the corresponding G is not present in the genome (mismatch). The mismatches derive from the demand that the first letter ought to be G for efficient in vitro transcription by T7 or SP6 polymerase. The numerals shown at the right indicate the locations of the corresponding sequences with respect to the prospective transcription start sites (the 5’end of the longest cDNA) for each gene
