Skip to main content

Table 2 Results of microinjections and screening of transgenic founders

From: Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering

  injected survived at 7dpf good expression survived to adult positive founder
vacht sg1 84 20 11 10 5 / 10 (50%)
nr5a1 sg1 123 31 3 1 1 / 1 (100%)
sox5 sg1 52 25 11 10 7/ 10 (70%)
pax7a sg1 75 32 9 7 6 / 7 (85.7%)
pnp4a sg1 237 69* 13 6 3 / 6 (50%)
total 571 177 47 34 23 / 34 (67.6%)
  1. The asterisk (*) indicates the number of surviving fish at 2 dpf