Table 2 Results of microinjections and screening of transgenic founders
From: Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
injected | survived at 7dpf | good expression | survived to adult | positive founder | |
|---|---|---|---|---|---|
vacht sg1 | 84 | 20 | 11 | 10 | 5 / 10 (50%) |
nr5a1 sg1 | 123 | 31 | 3 | 1 | 1 / 1 (100%) |
sox5 sg1 | 52 | 25 | 11 | 10 | 7/ 10 (70%) |
pax7a sg1 | 75 | 32 | 9 | 7 | 6 / 7 (85.7%) |
pnp4a sg1 | 237 | 69* | 13 | 6 | 3 / 6 (50%) |
total | 571 | 177 | 47 | 34 | 23 / 34 (67.6%) |