Fig. 3
From: Development of hypobranchial muscles with special reference to the evolution of the vertebrate neck
Trajectory of HBM precursors in mouse embryos. Transverse sections of mandibular, hyoid and branchial arch levels in Wnt1-Cre/R26R-H2B-EGFP embryos at stage E10.5. Bottom panels show the magnification of pharyngeal floor. Arrowheads indicate HBM precursors. PAX3 protein was detected on both sides of the EGFP-negative basibranchial mesenchyme and laterally surrounded by EGFP-positive neural crest cells at the hyoid arch. Bbm, basibranchial mesenchyme; cncc, cardiac neural crest cells; eb1–4, ectomesenchyme of first to fourth branchial arch; eh, ectomesenchyme of hyoid arch; em, ectomesenchyme of mandibular arch; ha, hyoid arch mesoderm; oft, outflow tract; ot, otic vesicle; ra, right atrium; thy thyroid gland. Scale bars, 50 μm