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Table 2 Summary of Illumina sequencing. Results based on the four PCR (blocking primer) settings are separately shown

From: DNA metabarcoding of spiders, insects, and springtails for exploring potential linkage between above- and below-ground food webs

Experiment

No. of Samples with reads

No. of Samples with Hexapoda reads

Samples with Hexapoda reads (%)

Mean no. reads per sample (Hexapoda)

Mean no. reads per sample (Araneae)

Total no. of reads (Hexapoda)

Total no. of reads (Araneae)

Percentage of Hexapoda reads (%)

Blocking primer A

200

30

15.0

271

3754

8130

750,862

1.08

Blocking primer B

204

25

12.3

445

3720

11,113

758,851

1.46

Blocking primers A & B

189

19

10.1

258

3985

4909

753,181

0.65

No blocking primers

207

19

9.2

335

3428

6363

709,647

0.90

Total

210

58

27.6

–

–

30,515

2,972,541

1.03