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Table 2 Summary of Illumina sequencing. Results based on the four PCR (blocking primer) settings are separately shown

From: DNA metabarcoding of spiders, insects, and springtails for exploring potential linkage between above- and below-ground food webs

Experiment No. of Samples with reads No. of Samples with Hexapoda reads Samples with Hexapoda reads (%) Mean no. reads per sample (Hexapoda) Mean no. reads per sample (Araneae) Total no. of reads (Hexapoda) Total no. of reads (Araneae) Percentage of Hexapoda reads (%)
Blocking primer A 200 30 15.0 271 3754 8130 750,862 1.08
Blocking primer B 204 25 12.3 445 3720 11,113 758,851 1.46
Blocking primers A & B 189 19 10.1 258 3985 4909 753,181 0.65
No blocking primers 207 19 9.2 335 3428 6363 709,647 0.90
Total 210 58 27.6 30,515 2,972,541 1.03