Fig. 4From: Ectopic Fgf signaling induces the intercalary response in developing chicken limb budsMitogenic activity was measured by pH 3 immunofluorescence. The left column shows the control samples with the PBS-soaked bead. The right column shows the limb buds with the Fgf2 + Fgf8-soaked bead. Asterisks and arrowheads indicate the beads and the ischemic region, respectively. The scale bar in A is 250 μm. (M) The summary of the result of A–L and the statistical analysis. n.s. = no significant differenceBack to article page