Fig. 2

Light micrographs of acinar dermal glands in the skin of T. verrucosus. a-c. Sections stained with Alcian blue at pH 2.5 and Periodic acid Schiff (AB-PAS) showing a the back region with large granular glands type 1 (GG1) extending deep into the dermis and a conspicuously smaller mucous gland (MG), b the dorsal tail edge with large granular glands type 2 (GG2) next to a MG and c details of a MG. Note that GG1s and GG2s neither react to the AB nor to the PAS test, while the MGs react both to AB and PAS (MG-lumen in C indicated by an asterisk). d-g. Toluidine blue stainings showing: d. Details of a MG with a central lumen (indicated by asterisk) surrounded by smaller cells in the apical half and large cells filled with secretory material in the basal half of the gland. e. Cross section through the dorsal tail edge, ca. 3 cm distal from the tail base showing the densely arranged giant granular glands type 2 (GG2). e and f. Details of GG1 (e) and GG2 (f) illustrating the differently arranged granules in the two gland types. H and I: Coomassie Brilliant Blue (CBB) stained sections from the lateral trunk (h) and ventral tail (i) showing positive CBB staining of GG1 (h) and GG2 (i) and negative CBB-reaction of MG (h). Ep, epithelium; De, dermis