Fig. 4From: A comprehensive comparison of sex-inducing activity in asexual worms of the planarian Dugesia ryukyuensis: the crucial sex-inducing substance appears to be present in yolk glands in TricladidaComparison of sex-inducing activity toward asexual worms of D. ryukyuensis. For the assay of the three fractions from the cytosolic fraction and EtOAc layer, test worms at the three stages of sexual induction identified by external observation were expressed as a doughnut chart. The number of test worms after the bioassay is shown in the center of a doughnut chart. The illustrations of worms in the figure correspond to those in Fig. 3b. The percentage of worms at each of the three stages of sexual induction is shown in the box drawn by colored lineBack to article page