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Table 1 PCR primers used for the expression analysis and the preparation of the templates for the cRNA probes used in in situ hybridization

From: RFamidergic neurons in the olfactory centers of the terrestrial slug Limax

Target cDNA

Upstream primer

Downstream primer

Region

Genbank acc. no.

aa. identitiy (%) to Deroceras gene

FMRFamide

agacgataagctccaccgctttg

tcttcagcctgaacgtatgacgc

83–300 (2–219)

AB546959 (AB546960)

79.1

LFRFamide1

atgatcaagagtcagctctcag

atcgctgtcactaccccagtg

15–563

LC375313

83.1

LFRFamide2

cgactctgataaaaggggctcc

atgtgactactgggaacgccag

566–1021

LC375314

70.6

CCK/SK1

cgctgcccacactgaacaag

ggtttcattgttgttggcgaag

743–1111

LC375319

73.5

CCK/SK2

agagcacaccactgaaggac

tcggatgtgtcggggttta

451–912

LC375320

82.4

NP-F1

gttgctctaataatttgctcgggag

gccgccatgtttgttagttgac

303–782

LC375315

91.7

NP-F2

caacacactacgcccacacag

ctgttcacgagtgtgctcgag

972–1520

LC375316

92.9

pedal peptide

atagaatcgctggtgcgtcgag

cgccgttatcgctgagttcatg

4219–4716

LC375323

FaRP-A

tgacgagctcgatacgacctatg

cctcgtggtcaaaccttctgaac

399–967

LC375321

FaRP-B

agaccagcgtgcgcaccag

ctgggccagcagacattgac

144–650

LC375322

luqin1

ccacccttaagtcagcaactc

ggaatagaaacacaggagagcgatg

401–1051

LC375317

88.7

luqin2

ctcttgtctcatctgcccacac

agctatccgaaccgtgtgagtc

19–552

LC375318

65.4

18S rRNA

gcttaccaagctccgaccctcgtgg

cgtcactacctccccgtgccggg

62–383

LC375324

  1. The accession numbers of the Limax RFamide cDNAs and the amino acid identities of precursors to the corresponding Deroceras homologues are shown in the right columns. The numerals in the “region” column indicate the amplified cDNA regions in the sequence data deposited in Genbank. The information for the splice variant of FMRFamide (FMRFamide Type II) is shown in parentheses