Fig. 9

Backfill experiments. a–d Backfill of the ultimate leg nerves N4 + N5. e, f Backfill of the walking leg 10 nerves N4 + N5. a Black/white inverted cLSM maximum projection of ultimate leg nerve backfill, view from dorsal, right hemiganglion. Note the intensely labeled motoneurons (mn) in the lateral cortex of G15. Scale bar 100 μm. b Backfill of ultimate leg nerve a depth color coded. The ventralmost area (purple) is characterized by the vnd (compare c). Scale bar 50 μm. c Subsection of b, the ventral neuropilar domain features three distinct glomerular subunits (asterisks). Scale bar 25 μm. d Depth color-coded 3D visualization (Amira) showing the dorso-ventral organization of N4 + N5 afferents, view from medial. N4 + N5 split into a dorsal anteriad projection (dap) and a ventral anteriad projection (vap). Both projections each branch off a small posteriad projection (dorsal and ventral posteriad projections, dpp and vpp). The vap splits into the ventral lamellate domain (vld) and the ventral neuropilar domain (vnd, asterisk). Scale bar 50 μm. e Black/white inverted cLSM maximum projection of waking leg nerve backfill, right hemiganglion. Note the intensely labeled neurons in the lateral and ventral cortex of G10. Scale bar 50 μm. f Depth color coded 3D visualization of backfill (e) showing the dorsoventral organization of N4 + N5 afferents, view from dorsomedial. The general branching pattern is similar to the ultimate leg-associated ganglion (compare d). However, the ventral anteriad projection is not differentiated into a lamellate domain, only giving rise to the ventral neuropilar domain (vnd, asterisk) which is far smaller in comparison. Scale bar 50 μm. Abbreviations: con connective, cu cuticle, dap dorsal anteriad projection, dpp dorsal posteriad projection, G ganglion of the vnc, mn motoneurons, N nerve, TG terminal ganglion, vap ventral anteriad projection, vld ventral lamellate domain, vnd ventral neuropilar domain, vpp ventral posteriad projection