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Table 2 Effect of temperature on the development of RNAi phenotypes

From: Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano

Temperature

Incubation time with dsRNA against Mlig-ddx39 gene until lethalitya, days, mean ± standard deviation

Percentage of animals with enlarged testesb after 4 days of incubation with dsRNA against Mlig-sperm1 gene

DV1

NL10

NL10

20 °C

17.8 ± 1.3

16.6 ± 1.9

0%

25 °C

10.1 ± 0.6

9.5 ± 0.5

25%

30 °C

7.9 ± 0.2

7.2 ± 0.4

85%

  1. ano lethality was observed at the tested temperatures when incubating animals with dsRNA against gfp as a control
  2. bwhen testes touch each other