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Table 2 Effect of temperature on the development of RNAi phenotypes

From: Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano

Temperature Incubation time with dsRNA against Mlig-ddx39 gene until lethalitya, days, mean ± standard deviation Percentage of animals with enlarged testesb after 4 days of incubation with dsRNA against Mlig-sperm1 gene
DV1 NL10 NL10
20 °C 17.8 ± 1.3 16.6 ± 1.9 0%
25 °C 10.1 ± 0.6 9.5 ± 0.5 25%
30 °C 7.9 ± 0.2 7.2 ± 0.4 85%
  1. ano lethality was observed at the tested temperatures when incubating animals with dsRNA against gfp as a control
  2. bwhen testes touch each other