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Fig. 5 | Zoological Letters

Fig. 5

From: Morphogenesis and development of midgut symbiotic organ of the stinkbug Plautia stali (Hemiptera: Pentatomidae)

Fig. 5

Morphogenesis of the symbiotic organ at early developmental stages of P. stali. a–e Bright-field images of the symbiotic organs. f–j Confocal microscopic images of the symbiotic organs, in which symbiotic bacteria are visualized by FISH in red and host’s nuclear DNA is counterstained in blue. a, f First instar nymph on the day of hatching. b, g First instar nymph 1 day after hatching. c, h First instar nymph 2 days after hatching. d, i First instar nymph 3 days after hatching. e, j Second instar nymph on the day of molting or 4 days after hatching. k–o Confocal microscopic images of the symbiotic organs of second instar nymphs 1 day after molting, where the levels of crypt formation and symbiont colonization exhibit considerable variation

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