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Fig. 6 | Zoological Letters

Fig. 6

From: Morphogenesis and development of midgut symbiotic organ of the stinkbug Plautia stali (Hemiptera: Pentatomidae)

Fig. 6

Morphogenesis of the symbiotic organ at late developmental stages of P. stali. a–d Bright-field images of the symbiotic organs. e–h FISH visualization of the symbiotic bacteria in red on tissue sections of the symbiotic organ, where host’s nuclear DNA is counterstained in blue. i-l Magnified images of (e-h). m–p Adjacent tissue sections to i–l stained with toluidine blue. a, e, i, m Fifth instar nymph 1 day after molting. b, f, j, n Fifth instar nymph 3 days after molting. c, g, k, o Fifth instar nymph 5 days after molting. d, h, l, p Fifth instar nymph 7 days after molting. In i-p, arrowheads highlight the crypts, asterisks show the main tract, two-way arrows indicate the crypt openings to the main tract, and arrows show the pedicel-like structures formed with the crypt closure

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