Fig. 2

Taste bud development of Amur catfish. Catfish embryos at 1 dpf (a, h, a′), 2 dpf (b, e, i, b′), 3 dpf (c, f, j, c′), and 4 dpf (d, g, k, d′) observed using fluorescent macroscopy. Lateral views are shown in a–k. Taste buds of the head (a–g) and trunk and tail (h-k) regions were visualized by anti-calretinin antibody (in red). Coronal sections of the oral cavity are shown in a′–d′. The dashed line in a–d indicates the cutting plane in a′–d′. Taste buds in the oral cavity are indicated by arrowheads. Blue staining shows nucleus labeled by DAPI. Insets in b, c, e and c′ indicate high magnification images of boxes in b, c, e and c′. adp, adipose fin; he, head region; li, lip; mdb, mandibular barbel; mxb, maxillary barbel; pf, pectoral fin; ta, tail region. Anterior is left in a–k. Scale bars in b–g, i–k and b′-d′ are 500 μm, and 200 μm in a, h and a′