Fig. 3

Development of peripheral nerves in Amur catfish. Peripheral nerve axons stained by anti-acetylated tubulin antibody are shown in in green. Blue staining shows nucleus labeled by DAPI. Lateral views are shown in a–d and e–j. Dorsal views are shown in a′–d′. Distribution of taste buds and innervation of peripheral nerves are shown in e–n. Arrowheads in i, j show maxillary barbels. Morphological relationships between taste buds and facial nerves on the barbel are shown in k–n. Peripheral nerve axons and taste buds were stained using anti-acetylated tubulin (in green) and anti-calretinin (in red) antibodies, respectively. Taste buds on catfish barbel are innervated by facial nerves and the nerve endings represent a specific “loop” (l, arrowheads). amdb, anterior mandibular barbel; mxb, maxillary barbel; hy, hyoid ramus of facial and anterior lateral line nerves; md, mandibular ramus of trigeminal and facial nerves; mx, maxillary ramus of trigeminal and facial nerves; op, ophthalmic ramus of trigeminal and facial nerves; pll, posterior lateral line nerve; pmdb, posterior mandibular barbel; rc, recurrent ramus of the facial nerve; sn, spinal nerves; Anterior is left in all images. Scale bars in a is 200 μm, 500 μm in b–j, and 50 μm in k–n