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Fig. 4 | Zoological Letters

Fig. 4

From: FGF- and SHH-based molecular signals regulate barbel and craniofacial development in catfish

Fig. 4

Development of cartilages and muscles in Amur catfish. Cartilages stained by Alcian blue are shown in a–d. Myosin filaments stained by immunohistochemistry using anti-MF20 antibody are shown in e–h (in red). Blue staining in e–h shows nucleus labeled by DAPI. Dorsal views are shown in a–d. Ventral views are shown in a′–d′. Lateral views are shown in e–h. Arrowheads in c, c′ and d′ indicate barbal cartilages. The inset in h indicates high magnification images of the box in h. ah, adductor hyomandibulae; amd, adductor mandibulae; bh, basihyal cartilage; ch, ceratohyal cartilage; do, dilator operculi; lap, levator arcus palatini; lt, levator tentacle; me, Meckel’s cartilage; pa, parachordal cartilage; pq, palatoquadrate cartilage; tr, trabecular cartilage. Anterior is the upper side in a–d and left in e-h. Scale bars are 1 mm in a–d, and 500 μm in e–h

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