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Fig. 7 | Zoological Letters

Fig. 7

From: FGF- and SHH-based molecular signals regulate barbel and craniofacial development in catfish

Fig. 7

Effects of SHH and FGF inhibitors on peripheral nerve morphology. Peripheral nerves (in green) were visualized by anti-acetylated tubulin antibody. Blue staining shows nucleus labeled by DAPI. ac are lateral and (df) are ventral. Cyclopamine-treated embryos (b, e) represented apparently normal distribution of peripheral nerves compared with the control group (a, d), although their mandibular regions became smaller and mandibular barbels were absent. However, with SU5402 treatment, peripheral nerve axons were poorly developed (c, f). gl effects of SHH and FGF inhibitors on the peripheral nerves in the maxillary (gi) and mandibular (jl) barbels of Amur catfish. Insets in g, h indicate high magnification images of boxes in g, h. In the control group at 5 dpf (g), peripheral nerves (mx and md) extended normally and reached the distal end of the barbel. In the cyclopamine-treated embryos, both mx and md entered into the maxillary and mandibular barbels and reached the distal end, consistent with the control group, although the length of the barbels shortened (h, k). However, with SU5402 treatment, the peripheral nerves failed to enter the barbels (i, l). ab, anterior mandibular barbel ramus of trigeminal and facial nerves; hy, hyoid ramus of facial and anterior lateral line nerves; md, mandibular ramus of trigeminal and facial nerves; mx, maxillary ramus of trigeminal and facial nerves; op, ophthalmic ramus of trigeminal and facial nerves; pb, posterior mandibular barbel ramus of trigeminal and facial nerves; rc, recurrent ramus of the facial nerve. Anterior is left in ac and the upper side in df. In g, left and upper sides show proximal and anterior sides, respectively. In h and i, left and upper sides show distal and anterior sides, respectively. In jl, upper and left sides show proximal and anterior sides, respectively. Scale bars in ag and j are 500 μm, 400 μm in H, and 200 μm in i, k, and l

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