Skip to main content
Fig. 6 | Zoological Letters

Fig. 6

From: Evolution of cis-regulatory modules for the head organizer gene goosecoid in chordates: comparisons between Branchiostoma and Xenopus

Fig. 6

Transgenic reporter analysis of the gsc 5′ region in Xenopus embryos. Transgenic reporter assays of the − 3 kb region of Xt_gsc and the − 4.5 kb region of Bj_gsc in Xenopus embryos. Panels represent whole mount in situ hybridization of the reporter gene mVenus (first and second rows) or the endogenous gsc gene (third row) in dorso-ventral hemisections. Expression patterns were examined at the early (st. 10), middle (st. 11) and late (st. 12.5) gastrula stages and late-neurula stage (st. 23) as indicated. In right panels of late gastrula embryos, hemisections were represented in the dorsal view with anterior to the top. Other panels of gastrula are shown with animal to the top and dorsal to the right. Neurula embryos are shown with dorsal to the top and anterior to the left. Neurula embryos cleared in BB/BA solution are shown in right panels. Arrowheads, blastopore; open arrowheads, mouth; and arrows, notochord. Additional file 1: Figure S2 shows results in more detail (see Additional file 1)

Back to article page