Fig. 3

Sequence analysis of 100-kDa proteins detected by mAb11B16B10. a 100-kDa proteins detected by mAb11B16B10 sequence compared with that of DD3–3-like protein from C. intestinalis (XP_002122731.1). Bold line: Identical sequence determined using Edman degradation. Square box: N-glycosylation site. b Schematic of 100-kDa proteins detected by mAb11B16B10. c Phylogenetic analysis of HrSMLP1 and DD3–3-like proteins. The maximum-likelihood tree was constructed using MEGA version 7.0.26. DD3–3 protein from the social amoeba Dictyostelium discoideum represent the outgroup. The scale bar indicates the number of substitutions per site. Numbers at the nodes indicate the percentages of 1000 bootstrap replications (when higher than 50%). Accession numbers: Crassostrea gigas 1 (XP_011412144.1), C. gigas 2 (XP_011412151.1), Pomacea canaliculata (XP_025107313.1), Capitella teleta 1 (ELT93314.1), C. teleta 2 (ELT93316.1), Saccoglossus kowalevskii 1 (XP_006822468.1), S. kowalevskii 2 (XP_006822467.1), Acanthaster planci (XP_022106441.1), Strongylocentrotus purpuratus 1 (XP_785293.2), S. purpuratus 2 (XP_801877.1), Trichoplax adhaerens 1 (XP_002111380.1), T. adhaerens 2 (XP_002111868.1), Branchiostoma floridae 1 (XP_002606693.1), B. floridae 2 (XP_002606694.1), Nematostella vectensis 1 (XP_001629203.1), N. vectensis 2 (XP_001634659.1), N. vectensis 3 (XP_001638964.1), N. vectensis 4 (XP_001628323.1), Amphimedon queenslandica 1 (XP_003383003.1), A. queenslandica 2 (XP_003383061.1), Ciona intestinalis1 (XP_002122731.1), C. intestinalis 2 (XP_002128670.1), Oikopleura dioica (CBY14070.1), Hydra vulgaris (XP_002165689.2), Dictyostelium discoideum (BAD93186.1)