Fig. 5

Morphology of primary neurites of histaminergic neurons in the ventral nerve cord of Xibalbanus tulumensis. Maximum projections of cLSM stacks (ventral view, anterior to the top). a At least two somata of the cluster of anterior cell bodies (AC) sent neurites (black arrows) into an elongated granular-structured region (black arrowheads), which is associated to the ipsilateral anterior segmental nerve (ASN) (black double arrowheads). b The medial cell bodies (MC) projected laterally toward the center of the ipsilateral hemiganglion (ganglionic midline indicated by dashed line). c The primary neurites of the posterior cell bodies (PC) projected anteromedially and crossed the midline. These neurites could be traced to the center of the contralateral hemiganglion until the signal was lost. d Besides the rather strongly immunoreactive posterior commissure (black arrow) originating in the PC, two faintly labeled contralateral axons could be identified anteriorly (black arrowheads). e Short medial projections associated to the laterocentral cell bodies could be identified (black arrows). f Reconstruction drawings of selected histaminergic neurons in a ganglion of the ventral nerve cord. Abbreviations: AC: anterior cell bodies; ASN: anterior segmental nerve; LCC: laterocentral cell bodies; MC: medial cell bodies; PC: posterior cell bodies; PSN: posterior segmental nerve. Scale bars: a–e: 25 μm