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Fig. 3 | Zoological Letters

Fig. 3

From: The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus

Fig. 3

Expression rhythms of cwo in the optic lobe of the cricket Gryllus bimaculatus and the effects of clock gene RNAi on the expression of cwo. a: cwo is rhythmically expressed in the optic lobe with a peak level at midnight in both untreated (gray) and DsRed2RNAi-treated crickets (black) under LD. dscwo strongly suppressed cwo mRNA levels and eliminated its rhythmic expression (orange). b: cwo is also rhythmically expressed under DD in the optic lobe of untreated (gray) and DsRed2RNAi-treated crickets (black). dscwo again suppressed cwo mRNA levels and eliminated its rhythmic expression (orange). c: Effects of dsRNA of clock genes, per, tim, Clk, cyc, and cry2 on cwo expression under LD. perRNAi, timRNAi, and cycRNAi eliminated the daily rhythmic expression of cwo, and the suppression was stronger in perRNAi- and cycRNAi-treated crickets. ClkRNAi and cry2RNAi did not eliminate the rhythm. cry2RNAi treatment had no significant effects on the expression rhythm of cwo, while ClkRNAi induced its phase shift to peak at ZT2. Asterisks indicate significant differences compared to the control treated with dsDsRed2 (* P < 0.05, ** P < 0.01, Dunnett’s test). Different lowercase letters indicate that the values differ significantly from each other (Tukey’s test, P < 0.05). White, gray, and black bars above the panel indicate light (white), subjective day (gray), and dark/subjective night (black) fractions, respectively. See text for details

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