Fig. 5
From: The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus
Effects of cwo dsRNA on the mRNA expression of clock genes per, tim, Clk, cyc, vri, Pdp1, E75, and HR3 under LD. Orange: cwoRNAi-treated crickets. Black: DsRed2RNAi-treated control crickets. Dscwo significantly downregulated per, Clk, and cyc and eliminated the daily rhythmic expression of per and cyc. Dscwo neither downregulated tim and cry2 nor eliminated their rhythmic expression but induced a slight phase delay. Vri, Pdp1, E75, and HR3 were significantly upregulated, and their expression stayed at a level near their peak level in DsRed2RNAi-treated controls or higher. Different letters indicate a significant difference detected by Tukey’s test (P < 0.05). Asterisks indicate significant differences compared to the control treated with dsDsRed2 (* P < 0.05, ** P < 0.01, t-test). White and black bars above the panel indicate light (white) and dark (black) fractions, respectively. See text for details