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Fig. 6 | Zoological Letters

Fig. 6

From: The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus

Fig. 6

Effects of cwo dsRNA on the mRNA expression of clock genes per, tim, Clk, cyc, vri, Pdp1, E75, and HR3 under DD. Orange: cwoRNAi-treated crickets. Black: DsRed2RNAi-treated control crickets. Dscwo significantly downregulated per, tim, Clk, cyc, and E75 and eliminated the daily rhythmic expression of per, tim, cyc, vri, and Pdp1. cry2 maintained its rhythmic expression. Different letters indicate a significant difference detected by Tukey’s test (P < 0.05). Asterisks indicate significant differences compared to the control treated with dsDsRed2 (* P < 0.05, ** P < 0.01, t-test). Gray and black bars above the panel indicate subjective day (white) and subjective night (black), respectively. See text for details

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