A new redescription of Richtersius coronifer, supported by transcriptome, provides resources for describing concealed species diversity within the monotypic genus Richtersius (Eutardigrada)

Table 2 Primers and references for PCR protocols for amplification of the four DNA fragments sequenced in the study

DNA marker	Primer name	Primer direction	Primer sequence (5′-3′)	Primer source	PCR programme
18S rRNA	18S_Tar_Ff1	forward	AGGCGAAACCGCGAATGGCTC	[57]	[58]
18S rRNA	18S_Tar_Rr1	reverse	GCCGCAGGCTCCACTCCTGG	[57]	[58]
28S rRNA	28S_Eutar_F	forward	ACCCGCTGAACTTAAGCATAT	[59, 60]	[60]
28S rRNA	28SR0990	reverse	CCTTGGTCCGTGTTTCAAGAC	[59, 60]	[60]
ITS-2	ITS2_Eutar_Ff	forward	CGTAACGTGAATTGCAGGAC	[7]	[7]
ITS-2	ITS2_Eutar_Rr	reverse	TCCTCCGCTTATTGATATGC	[7]	[7]
COI	COI_Para_F	forward	TTTCAACAAACCACAAAGATATYGG	[61]	[9]
	COI_Eutar_Rr	reverse	TAAACTTCTGGGTGACCRAARAAYCA	[61]
	LCO1490	forward	GGTCAACAAATCATAAAGATATTGG	[62]
	COI_Mac_Rr	reverse	TGTTGGTATARAATWGGGTC	[101]

ISSN: 2056-306X