Fig. 2

DiI-labeled head cavities at the pharyngula stage. a-b′ An embryo with DiI injected into the prmc at st. 25. a Left lateral view of the DiI-injected embryo at 0.5 days postinjection (dpi). b-b′ A sagittal section of (a) and a magnified image in the inset (b′) showing DiI-labeled cells (magenta) found specifically in the epithelial wall of the prmc. c-d′ An embryo with DiI injection into the hyc at st. 25. c Left lateral view of the DiI-injected embryo at 0.5 dpi. (d-d′) A sagittal section of (c) and a magnified image in the inset (d′) show DiI-labeled cells (magenta) found specifically in the epithelial wall of the hyc. e-f′ An embryo with DiI injection into the mnc at st. 25. e Left lateral view of a DiI-injected embryo at 0.5 dpi. f-f′ A sagittal section of (e) and a magnified image in the inset (f′) show DiI-labeled cells (magenta) found specifically in the epithelial wall of the mnc. Panels b, b′, d, d′, f, and f′ show sections counterstained with DAPI (gray). hyc, hyoid head cavity; mnc, mandibular head cavity; prmc, premandibular head cavity. Scale bars = 500 μm (a, b, c, d, e, and f), 200 μm (b′, d′, and f′)