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Table 1 Dr-SLC38A9 RNAi knockdown during sexual induction

From: d-Tryptophan enhances the reproductive organ-specific expression of the amino acid transporter homolog Dr-SLC38A9 involved in the sexual induction of planarian Dugesia ryukyuensis

Treatment period Test fooda Number of worms developing a pair of ovaries (%)b Number of worms developing copulatory apparatus (%)b Number of worms developing a genital pore (%)b
Week 4 EGFP dsRNA 29/29 (100) 21/29 (72.4) 0/29 (0)
Dr-SLC38A9 dsRNA 22/29 (75.9)*c 5/29 (17.2)**d 0/29 (0)
Week 6 EGFP dsRNA 18/18 (100) 18/18 (100) 14/18 (77.8)
Dr-SLC38A9 dsRNA 19/19 (100) 15/19 (78.9) n.s.e 4/19 (21.1)***f
  1. aAsexual worms were fed minced B. brunnea worms supplemented with EGFP (control), daily or Dr-SLC38A9 dsRNA for up to 6 weeks (summarized in Fig. 4)
  2. bDevelopment of ovaries, copulatory apparatus, and a genital pore were observed externally using a stereoscopic microscope
  3. cStatistical significance was calculated using one-sided Fisher’s exact test (*P = 0.0052)
  4. dStatistical significance was calculated using the Chi-squared test (**х2 (1, N = 58) = 17.8, P < 0.001)
  5. eStatistical significance was calculated using Fisher’s exact test (P = 0.059, n.s.: not significant)
  6. fStatistical significance was calculated using the Chi-squared test with Yates’ continuity correction (***х2 (1, N = 37) = 9.7, P = 0.0018)