Table 2 Primers with their original references used for amplification of the four DNA fragments sequenced in the study. The primer set LCO1490-JJ + HCO2198-JJ was used for COI amplification in four populations (FI. 066, IT. 337, PL. 015, US. 057), whereas LCO1490 + HCOoutout was used for one population (ME.007)
DNA marker | Primer name and source | Primer direction | Primer sequence (5’-3’) |
|---|---|---|---|
18S rRNA | 18S_Tar_Ff1 [35] | forward | AGGCGAAACCGCGAATGGCTC |
18S_Tar_Rr1 [35] | reverse | GCCGCAGGCTCCACTCCTGG | |
28S rRNA | 28S_Eutar_F [36] | forward | ACCCGCTGAACTTAAGCATAT |
28SR0990 [37] | reverse | CCTTGGTCCGTGTTTCAAGAC | |
ITS-2 | ITS2_Eutar_Ff [38] | forward | CGTAACGTGAATTGCAGGAC |
ITS2_Eutar_Rr [38] | reverse | TCCTCCGCTTATTGATATGC | |
COI | LCO1490-JJ [39] | forward | CHACWAAYCATAAAGATATYGG |
HCO2198-JJ [39] | reverse | AWACTTCVGGRTGVCCAAARAATCA | |
LCO1490 [40] | forward | GGTCAACAAATCATAAAGATATTGG | |
HCOoutout [41] | reverse | GTAAATATATGRTGDGCTC |